Validation of pyrithiobac sodium (Staple herbicide) ELISA for Australian cotton soils

An enzyme-linked immunosorbent assay (ELISA) for pyrithiobac-sodium (Staple) produced by DuPont was validated in Australian soils. This pyrithiobac-sodium ELISA was shown to be highly sensitive with the limit of detection of 4-5 ppt. Soil samples were extracted either in PBS buffer by shaking or by accelerated solvent extraction (ASE). While pyrithiobac sodium can be analyzed directly by ELISA after ASE extraction with 1/10 or more dilutions, the analysis of PBS extract required filtration and dilution 1/20 or more depending on the concentration. Immunoassay results compared favorably with GC-MS results for both ASE and PBS extract of incurred residue of pyrithiobac sodium in soil samples, indicating that this ELISA can be an inexpensive and reliable alternative to conventional residue analysis methods for quantification of pyrithiobac-sodium. This validation provided the basis for applying the ELISA to a field study of pyrithiobac-sodium.     

Antimony bioavailability in mine soils

Five British former mining and smelting sites were investigated and found to have levels of total Sb of up to 700 mg kg(-1), indicating high levels of contamination which could be potentially harmful. However, this level of Sb was found to be biologically unavailable over a wide range of pH values, indicating that Sb is relatively unreactive and immobile in the surface layers of the soil, remaining where it is deposited rather than leaching into lower horizons and contaminating ground water. Sb, sparingly soluble in water, was unavailable to the bacterial biosensors tested. The bioluminescence responses were correlated to levels of co-contaminants such as arsenic and copper, rather than to Sb concentrations. This suggests that soil contamination by Sb due to mining and smelting operations is not a severe risk to the environment or human health provided that it is present as immobile species and contaminated sites are not used for purposes which increase the threat of exposure to identified receptors. Co-contaminants such as arsenic and copper are more bioavailable and may therefore be seen as a more significant risk.     

Collaborative networks and new ways of knowing

To move beyond legal and regulatory gridlock around water issues in the California Bay-Delta, a new inter-agency initiative, commonly known as CALFED, was created in 1994. CALFED has been an ongoing experiment in policy innovation. Part of the change in management practice has involved constructing new arenas that engage multiple perspectives and transform regulatory impasse into provisional steps forward. We examine the construction of so-called boundary objects, which are forums and policy instruments that cross group boundaries and foster integrative deliberation. We compare the design and action of two boundary objects created by CALFED, namely the Environmental Water Account (EWA) and the Water Use Efficiency (WUE) program. We find that the presence of the boundary object, in itself, does little to explain the success of each policy experiment. Rather, the answer lies in the types of network interactions that result, along with the way meaning is coproduced. In fact, rather than create new patterns of interrelationship (e.g., between fish habitat advocates and pump station operators), the boundary object might further embed institutionalized routines. To more deeply understand what makes the new institution an integrative one, we introduce the concept of Ways of Knowing which explains how new knowledge emerges from the network of new relationships.     

The toxicological paradigms of aluminum and the etiology of Alzheimer's disease in the nematode Caenorhabditis elegans

This study examined behavioral and enzymatic changes of C. elegans from its exposure to aluminum, and the resulting relationship with Alzheimer's disease. After chronic and acute exposure to aluminum, the results indicated that it alters the cholinergic status and behavior parameters of the nematode, suggesting a relationship between exposure to aluminum and the etiology of AD.     

Factors affecting conviction rates of intoxicated driver patients in two large trauma centers

Objective: Outcomes from previous studies report the driving under the influence (DUI) conviction rates for trauma patients in several cities within Canada and the United States over the last 2 decades. This study reports charge, conviction, and prosecution rates for trauma patients at 2 level I trauma centers servicing a large metropolitan city.

Methods: A retrospective review of the trauma databases was completed to identify patients meeting inclusion criteria. Four hundred sixty patients were identified and their records were compared with the district attorney's records for DUI charges and convictions.

Results: The conviction rate for this study was 8.7%, demonstrating continued low rates of conviction despite growing interest and public awareness of drinking and driving.

Conclusions: We discuss legal considerations that providers should consider when treating patients who have been drinking and driving.     

The victims of unsustainability: a challenge to sustainable development goals

Environmental unsustainability is due to both structural features and historically specific characteristics of industrial capitalism resulting in specific patterns of production and consumption, as well as population growth. Sustainability literature criticises the established corporate and political power hegemonies, interested in maintaining economic growth, as well as inability or unwillingness of citizen-consumers to counteract these hegemonic tendencies. Yet, official policies are still targeted at social and economic ‘development’ as a panacea for unsustainability challenges. Instead, renewed accent on social and economic objectives are outlined by a set of sustainable development goals (SDG) that include objectives of fighting poverty, promoting better health, reducing mortality, and stimulating equitable economic growth. What is less commonly critiqued is the underlying morality of unsustainability and ethical questions concerned with the ‘victims of unsustainability’ outside of socioeconomic discourse. The achievement of SDG goals, as will be further elaborated on in this article, is unlikely to lead to greater social equality and economic prosperity, but to a greater spread of unsustainable production and consumption, continuous economic as well as population growth that has caused environmental problems in the first place and further objectification of environment and its elements. This article argues that an invocation of ethical duty toward environment and its elements is required in order to move beyond the current status quo. Such ethical approach to unsustainability can effectively address the shortcomings of the mainstream sustainability discourse that is mainly anthropocentric and therefore fails to identify the correct locus of unsustainability.     

Health and population-dependent effects of ocean acidification on the marine isopod Idotea balthica

Three populations of the grazing isopod Idotea balthica were exposed to high CO₂ treatment for a period of 20 days to investigate the effect of ocean acidification (OA) on animal health and immunocompetence. The results of the populations from more saline habitats were comparable and showed a 60–80 % decrease in immune response as a result of the high CO₂ treatment. Analysis of protein carbonyls showed no treatment effect, indicating that short-term OA does not increase oxidative protein damage. Meanwhile, the third tested population from the lower saline Baltic Sea had higher background protein carbonyl levels. Ocean acidification in addition to this resulted in 100 % mortality. The results of this study show that OA reduced immunocompetence of this marine isopod. In addition, populations and individuals in poor health are potentially at greater risk to succumb under OA.     

Investigation of naphthalene contamination in olive oil from Greece

Abstract

Incidents of using naphthalene in olive orchards as a repellent of olive fly (Bactrocera oleae) have recently been recorded. Naphthalene, the primary ingredient of mothballs, is described to be diluted in plastic bottles and hanged on the trees releasing its characteristic odor which is supposed to repel olive fly. Since naphthalene has been characterized as possibly carcinogenic to humans and animals, it was considered essential to monitor olive oils regarding their potential contamination with this specific pollutant. To this goal, a simple, sensitive and reliable analytical method for the determination of naphthalene in olive oil has been developed. The method involves extraction with ethyl acetate: acetonitrile (1:1) and determination of naphthalene using gas chromatography triple quadrupole mass spectrometry. The method was validated in four fortification levels, 0.005, 0.01, 0.05, and 0.1?mg/kg in accordance with the EU requirements. The obtained results were acceptable as far as validation criteria are concerned, given recoveries between 71% and 87% and RSDs between 5% and 24%. The limit of determination for naphthalene was set at 0.005?mg/kg based on the lowest concentration level being validated with acceptable accuracy. The analytical technique was successfully applied to 75 olive oil samples collected from oil mills in Greece and no positive results of naphthalene were detected.